High spatial resolution nanoslit SERS for single-molecule nucleobase sensing
نویسندگان
چکیده
منابع مشابه
Supplementary information for “Bi-Analyte Single Molecule SERS with Simultaneous Spatial Resolution” Determination of the spatial resolution of the CCD
FIG. S1: (a) Schematic representation of the spectrometer. The laser spot on the sample is amplified by a fixed magnification factor of the collecting optics onto the entrance slit of the spectrometer; which in our machine is the same as the confocal pinhole and it is computer controlled. The magnification of the collecting optics in our system for a ×100 objective is X = 56. The Raman signal o...
متن کاملSingle-molecule high-resolution imaging with photobleaching.
Conventional light microscopy is limited in its resolving power by the Rayleigh limit to length scales on the order of 200 nm. On the other hand, spectroscopic techniques such as fluorescence resonance energy transfer cannot be used to measure distances >10 nm, leaving a "gap" in the ability of optical techniques to measure distances on the 10- to 100-nm scale. We have previously demonstrated t...
متن کاملSilicon nanowire based single-molecule SERS sensor.
One-dimensional nanowire (NW) optical sensors have attracted great attention as promising nanoscale tools for applications such as probing inside living cells. However, achieving single molecule detection on NW sensors remains an interesting and unsolved problem. In the present paper, we investigate single-molecule detection (SMD) on a single SiNW based surface-enhanced Raman scattering (SERS) ...
متن کاملHigh-Resolution Optical Tweezers for Single-Molecule Manipulation
Forces hold everything together and determine its structure and dynamics. In particular, tiny forces of 1-100 piconewtons govern the structures and dynamics of biomacromolecules. These forces enable folding, assembly, conformational fluctuations, or directional movements of biomacromolecules over sub-nanometer to micron distances. Optical tweezers have become a revolutionary tool to probe the f...
متن کاملA high-resolution magnetic tweezer for single-molecule measurements
Magnetic tweezers (MT) are single-molecule manipulation instruments that utilize a magnetic field to apply force to a biomolecule-tethered magnetic bead while using optical bead tracking to measure the biomolecule's extension. While relatively simple to set up, prior MT implementations have lacked the resolution necessary to observe sub-nanometer biomolecular configuration changes. Here, we dem...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2018
ISSN: 2041-1723
DOI: 10.1038/s41467-018-04118-7